 COA |
MSDS |
 HPLC |
NMR |
| CAS No: | 483367-10-8 |
| Molecular formula(MF) | C31H32N6O2 |
| Molecular Weight(MW): | 520.62 |
| Alias | PURMORPHAMINE;9-Cyclohexyl-N-[4-(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)-9H-purin-6-amine;9-Cyclohexyl-N-[(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)-9H-purin-6-amine |
| In vitro | DMSO | 4 mg/mL warmed (7.68 mM) |
|---|---|---|
| Water | <1 mg/mL | |
| Ethanol | <1 mg/mL | |
| In vivo | 2% DMSO+30% PEG 300+5% Tween 80+ddH2O | 1mg/mL |
| Description | Purmorphamine, which directly binds and activates Smoothened, blocks BODIPY-cyclopamine binding to Smo with IC50 of ~ 1.5 μM in HEK293T cell and also is an inducer of osteoblast differentiation with EC50 of 1 μM. | ||||||||||||||||||||||||||||||
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| In vitro |
Purmorphamine activates the Hedgehog pathway by directly binds and activates Smoothened with IC50 of ~ 1.5 μM in compete with cyclopamine, a Smo antagonist. [1] Purmorphamine is a potent inducer of osteogenesis in multipotent C3H10T1/2 cells. The EC50 (based on ALP expression) for Purmorphamine is 1 μM in C3H10T1/2 cells. Purmorphamine (1 μM) and BMP-4 (100 ng/mL) together increase ALP activity more than 90-fold in 3T3-L1 cells. [2] In contrast to BMP-4, Purmorphamine induces osteogenesis by activating Hedgehog signaling in multipotent mesenchymal progenitor cells. [3] |
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| Cell Data |
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| In vivo | Purmorphamine up-regulates ALP expression in human mesenchymal stem cell-based constructs on rats. [4] |